Exopolysaccharides (EPS) have largely been studied for their diverse structural properties and potential applications in biotechnology. Particularly those produced by bacteria isolated from phyllosphere, the aerial surfaces of plants, play critical roles in bacterial adhesion, desiccation tolerance, and biofilm formation, contributing to both microbial survival and plant health. Typical conditions promoting EPS production include osmotic stress, drought, and exposure to pollutants or heavy metals that trigger enhanced EPS synthesis as a protective mechanism. EPS biosynthesis typically follows well-characterized biosynthetic pathways and are regulated by complex genetic and environmental signals. In this work, we studied two bacterial isolates from olive leaves affiliated with Rhodococcus and Halomonas and reconstructed their EPS biosynthetic pathways using a genome mining approach. While Rhodococcus sp 24CO follows the Wzx/Wzy-dependent pathway, which involves the polymerization and translocation of repeat units via specific flippases and polymerases, Halomonas sp. 44HA contains the genes involved in the ABC transporter-dependent pathway, where the polysaccharide is synthesized in the cytoplasm and exported across the membrane by ATP-binding cassette transporters. Growth conditions promoting EPS production were also studied. We are currently working on on their possible biotechnological applications that could benefit different central industries in Argentina, such as petroleum refineries and agriculture.